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Why is Masson trichrome used?

By: Manuel RapinoUpdated: February 14, 2021

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Standard applications: Masson's trichrome staining is widely used to study muscular pathologies (muscular dystrophy), cardiac pathologies (infarct), hepatic pathologies (cirrhosis) or kidney pathologies (glomerular fibrosis). It can also be used to detect and analyze tumors on hepatic and kidney biopsies.

Herein, what does trichrome stain for?

Trichrome staining is used to visualize connective tissues, particularly collagen, in tissue sections. In a standard Masson's Trichrome procedure, collagen is stained blue, nuclei are stained dark brown, muscle tissue is stained red, and cytoplasm is stained pink.

Furthermore, what color does collagen stain?

Collagen fibers stain green or blue with Masson's trichrome stain. Muscle and keratin will be red. Cytoplasm will be pink to red.

How does Masson's trichrome work?

Masson Trichrome Staining
Weigert's iron hematoxylin stains the nuclei in black, Biebrich scarlet-acid fuchsin stains cytoplasm & muscle fibers in red and after treatment with phosphotungstic and phosphomolybdic acid, collagen is stained in blue with aniline blue. Staining procedure: 1.

Is the nucleus basic or acidic?

DNA (heterochromatin and the nucleolus) in the nucleus, and RNA in ribosomes and in the rough endoplasmic reticulum are both acidic, and so haemotoxylin binds to them and stains them purple. Some extracellular materials (i.e. carbohydrates in cartilage) are also basophilic.

Related

What does Alcian blue stain for?

The alcian blue stain is most commonly used on tissue samples obtained from the gastrointestinal (GI) tract and is useful in diagnosing pathological processes such as Barrett's esophagus. Using alcian blue solutions of varying pH (1.0 and 2.5) also helps differentiate various types of acid mucosubstances.

What does light green stain?

Light green SF yellowish is a green triarylmethane dye that is used in the preparation of the staining solution which is widely used as a counterstain. Light green SF yellowish is also available as sterile strips used as a diagnostic agent when superficial corneal or conjunctival tissue change is suspected.

What type of stain is Giemsa stain?

Giemsa stain is a type of Romanowsky stain, named after Gustav Giemsa, a German chemist who created a dye solution. It was primarily designed for the demonstration of malarial parasites in blood smears, but it is also employed in histology for routine examination of blood smear.

What does reticulin stain?

The reticulin stain is extensively used in the histopathology laboratory for staining liver specimens, but can also be used to identify fibrosis in bone marrow core biopsy specimens. This silver staining process is known as silver impregnation.

What does haematoxylin stain?

Hematoxylin has a deep blue-purple color and stains nucleic acids by a complex, incompletely understood reaction. Eosin is pink and stains proteins nonspecifically. In a typical tissue, nuclei are stained blue, whereas the cytoplasm and extracellular matrix have varying degrees of pink staining.

What does silver stain stain?

Silver staining is the most sensitive colorimetric method for detecting total protein. The technique involves the deposition of metallic silver onto the surface of a gel at the locations of protein bands. Silver ions (from silver nitrate in the staining reagent) interact and bind with certain protein functional groups.

What is a common stain to demonstrate fibrosis in liver sections?

Liver fibrosis is well appreciated histologically on hematoxylin-eosin sections or with histochemical stains (Masson's trichrome or Sirius Red), which show collagen deposition with varying degrees of architectural distortion.

What does the Masson trichrome stain highlight in a liver biopsy?

Trichrome Stain
The stain imparts a blue color to collagen against a red background of hepatocytes and other structures. It stains type 1 collagen that is normally present in the portal tracts and vessel walls, but also highlights the presence and distribution of reactive fibrosis as a result of liver injury.

Does PAS stain collagen?

It is the basis of the PAS stain. PAS stains carbohydrates and carbohydrate rich macromolecules a deep red colour (magenta). Mucus in cells and tissues, Basement membranes, and Brush borders of kidney tubules and small and large intestines Reticular fibres (i.e. collagen) in connective tissue and Cartilage.

Why cells are stained?

The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.

What color do muscles acquire in Masson trichrome staining?

Masson Trichrome Staining
Weigert's iron hematoxylin stains the nuclei in black, Biebrich scarlet-acid fuchsin stains cytoplasm & muscle fibers in red and after treatment with phosphotungstic and phosphomolybdic acid, collagen is stained in blue with aniline blue. Staining procedure: 1.

What is a silver stain used for?

Silver staining is the use of silver to selectively alter the appearance of a target in microscopy of histological sections; in temperature gradient gel electrophoresis; and in polyacrylamide gels.

What are special stains in histology?

Special Stains. Hemotoxylin & Eosin (H & E) – The most widely used stain in medical diagnosis. This particular stain acts as the basis upon which further stains may be requested to confirm diagnosis. Nuclear proteins stain purple whereas cytoplasm and other tissue elements stain orange/red.

Does eosin stain collagen?

Eosin stains the cytoplasm and some other structures including extracellular matrix such as collagen in up to five shades of pink. The eosinophilic (substances that are stained by eosin) structures are generally composed of intracellular or extracellular proteins.

What Colour does PAS stain?

The Schiff reagent is a bleached basic fuschin that reacts with aldehyde groups. This reaction results in a deep red colour in the section. It is the basis of the PAS stain. PAS stains carbohydrates and carbohydrate rich macromolecules a deep red colour (magenta).

What are the three types of connective tissue fibers?

Connective Tissue Fibers and Ground Substance
Three main types of fibers are secreted by fibroblasts: collagen fibers, elastic fibers, and reticular fibers.