Hybridoma is a culture of hybrid cells that results from the fusion of B cells and myeloma cells. Hybridoma technology produces hybridomas. This technology was developed to produce mAbs. Hybridomas possess two important properties of B cells, production of antibodies, and immortalization of myeloma cells.
Also know, how hybridoma cells are produced?
Hybridomas are produced by injecting a specific antigen into a mouse, collecting an antibody-producing cell from the mouse's spleen, and fusing it with a tumor cell called a myeloma cell. The hybridoma cells multiply indefinitely in the laboratory and can be used to produce a specific antibody indefinitely.
Also, what cells are fused to make hybridoma cells?
The spleen cells are fused with human cancerous white blood cells called myeloma cells to form hybridoma cells which divide indefinitely. These hybridoma cells divide and produce millions of monoclonal antibodies specific to the original antigen.
Why myeloma cells are used in hybridoma?
Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies). The myeloma cell line that is used in this process is selected for its ability to grow in tissue culture and for an absence of antibody synthesis.
What is hybridoma give their example?
Hybridoma: A hybrid cell used as the basis for the production of antibodies in large amounts for diagnostic or therapeutic use. Hybridomas are produced by injecting a specific antigen into a mouse, collecting an antibody-producing cell from the mouse's spleen, and fusing it with a tumor cell called a myeloma cell.