Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to a green-fluorescent calcein after acetoxymethyl ester hydrolysis by intracellular esterases.
Also, is calcein AM toxic?
Calcein, AM has been used for studies of enzymatic activity, cell membrane integrity, and long-term cell tracking due to its low cellular toxicity. It is important to note that for certain cell lines and experimental conditions, relatively high concentrations of Calcein, AM can be toxic to cells.
Likewise, is calcein light sensitive?
Calcein AM Dye is supplied at ambient temperature. Upon arrival store at -20°C. The product when stored as directed is stable for 1 year. light as Calcien AM dye is light sensitive.
What is Trypan blue used for?
Trypan blue is a cell impermeant stain used to estimate the number of dead cells in a viable population. Its utility is based on the fact that it is a charged dye and does not enter cells unless the membrane is compromised.
How long does calcein AM last?