Principle: Just like widefield or confocal fluorescence microscopy, two-photon microscopy is based on fluorophore excitation, which results in the emission of light. In this process, photons combine their energy, which allows low-energy infrared photons to excite standard fluorophores, such as GFP.
Accordingly, what is two photon polymerization?
Two-photon polymerization is a non-linear optical process based on the simultaneous absorption of two photons in a photosensitive material (photoresist). This process changes the photosensitive material, i.e. it leads to a polymerization by activating so-called photo-initiators in the resist.
Also, what is two photon cross section?
February 2009) Two-photon absorption (TPA) is the absorption of two photons of identical or different frequencies in order to excite a molecule from one state (usually the ground state) to a higher energy, most commonly an excited electronic state.
What is two photon calcium imaging?
Two-photon calcium imaging is a powerful means for monitoring the activity of distinct neurons in brain tissue in vivo. In vivo two-photon Ca2+ recordings, obtained by imaging through the intact skull, indicated that whisker deflection-evoked Ca2+ transients occur in a subset of layer 2/3 neurons of the barrel cortex.
What is two photon microscopy used for?
Two-photon microscopy (also called multiphoton microscopy) can be used for live cell imaging of thick biological specimens, as it has several advantages over confocal microscopy. Molecules can be visualized deeply within the specimen with a maximal penetration depth of about 1 mm.